Characterization of a microbial isolate with Saccharifying action on coconut endosperm residue

Date

10-1988

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ernesto J. Del Rosario

Abstract

Limbo, Ma. Rowena Magno, College of Arts and Sciences, University of the Philippines at Los Banos, October, 1988. Characterization of a Microbial Isolate with Saccharifying Action on Coconut Endosperm Residue.

Adviser : Dr. Ernesto J. del Rosario

The characterization and identification of the principal microbial isolate (Gram-negative, rod-shaped bacteria) using cultural, morphological and physiological tests showed it to be Pseudomonas lemoignei. The saccharifying action on coconut endosperm residue "sapal" of two bacterial isolates (a rod-shaped, Gram-negative isolate and a Gram-positive coccus) were studied. The bacteria were inoculated as pure cultures and as mixed culture to the substrate at pH 5, 6, 7 and 8. Saccharification was studied by analyzing the protein and oil contents of the extracts after incubating the microbial suspension at room temperature (29°C) for five days in a gyrotatory shaker the highest protein and oil contents were obtained at pH 8 for all types of inoculum; significant differences in protein and oil extraction were observed at various pH values, No significant differences in protein and oil contents were observed using different types of inoculum.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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