Cytological effects of oregano, Coleus amboinicus lour (Dicotyldonae : Lamiales) leaf extract on human leukocyte chromosomes cultured in vitro

Date

3-1997

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Genaleen Q. Diaz

Abstract

LIMPOCO„ANNA GUIA 0. University of the Philippines Los Banos, BS Biology, MARCH 1997. Cytological Effects of oregano, Coleus amboinicus Lour (Dicotyldonae:Lamiales) Leaf Extract on Human Leukocyte Chromosomes Cultured in vitro.

Adviser Ma. Genaleen Q. Diaz

Leukocytes from two male volunteers were separately treated with 1,3, and 5 percent leaf extract of oregano, Coleus amboinicus Lour. Increased concentrations of leaf extract resulted to a decrease in mitotic index. Statistical analysis showed significant differences among the mitotic indices of the different treatments at 59,6 level of significance (13% in the control is reduced to 6.3%). The percentage of normal cells in the control, 1%, 3%, and 5% oregano leaf extract concentrations are not significantly different from one another. But, the percentage of cells with aberrations treated with 1%, 3%, and 5% oregano leaf extract concentrations is significantly different from the control. Statistical analysis of the frequencies of aberrations showed no significant differences among the treatments for gaps, significant difference at high concentration (5%) for loose sister chromatid pairs. But for breaks significant difference among treatments were observed. In all the treatments, breaks had the highest frequency followed by gaps and lastly loose sister chromatid pairs. Results showed that the oregano leaf extract is a probable chromosome breaking agent.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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