Genetic diversity analysis of Amaranthus spp. using Random Amplified Polymorphic DNA polymerase chain reaction (RAPD-PCR)

Date

4-2004

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Carmina C. Manuel

Abstract

LOGROÑO, AL-MARIE GRACE TINGSON. 2004. Genetic Diversity Analysis of Amaranthus spp. using Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR). University of the Philippines at Los Banos. B.S. Biology major in Cell Biology. Advisers: Prof Ma. Carmina C. Manuel and Dr. Takanori Sato ABSTRACT Seventy-seven (77) Amaranthus spp. from 15 countries collected in the Genetic Resource and Seed Unit of The Asian Vegetable Research and Development Center (AVRDC) were evaluated for their genetic diversity using RAPD-PCR assay. Thirty-five out of 47 random primers generated were used in the assay. A total of 618 RAPD bands were scored for all accessions with the average of 17.66 bands per primer. No species specific and country specific markers were observed. Primer OPD-08 generated the most number of bands (29) while Primer OPD-10 generated the least number of bands (9). Degree of polymorphism for each species was obtained based on the number of polymorphic bands scored. The data showed that A. mangostanus accessions contained the smallest variation with a percent polymorphism of 40.00%, followed by A. caudatus (40.4%). A. viridis accessions contained the highest variation (95.94%), followed by A. tricolor (90.09%), A. dubius (87.6%), A. spinosus (84.68%), and A. hypochondriacus (74.27%). The values obtained indicate a relatively high genetic diversity in the Amaranthus group of indigenous vegetables. Cluster analysis grouped the accessions into two major clusters containing seven subclusters with the truncation at 0.15 based on Jaccard's Similarity Coeffecient. Cluster A, which contains four subclusters, is composed of the A. dubius, (Al), A. spinosus, and A. atropurpureus (A2), A.hypochondriacus (A3), and Amaranthus sp. from Middle Asia (A4). Cluster B, which contains three subclusters, is composed of A. tricolor (B1), A. mangostanus (B2), and A. viridis (B3). As for the 18 unknown accessions, their possible identity was determined based on the dendrogram generated.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

This document is currently not available here.

Share

COinS