Genetic diversity in three species of chili pepper Capsicum (solanaceae) from Southeast Asia based on SDS- page of seed proteins

Date

3-2000

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ivan Marcelo A. Duka

Co-adviser

Liwayway M. Engle

Abstract

Lopez, Marijo Anne A. March 2000. University of the Philippines at Los Banos, Laguna. GENETIC DIVERSITY IN THREE SPECIES OF CHILI PEPPER CAPSICUM (SOLANACEAE) FROM SOUTHEAST ASIA BASED ON SDS-PAGE OF SEED PROTEINS.

Adviser: Prof. Ivan Marcelo A. Duka Co-adviser: Dr. Liwayway M. Engle

This study was conducted to determine the degree of genetic diversity among three species of Capsicum collected from Southeast Asia, namely: C annuum L., C. chinense Jacq., and C. frutescens L. using SDS-PAGE of seed proteins. Specifically, the study determined the SDS-PAGE seed protein bands and banding patterns, determined the geographic distribution of the bands and banding patterns, calculated the relative frequency of occurrence of specific hands and banding patterns species, computed for the percentage similarities (similarity index) within and between populations and inferred taxonomic relationship among the three Capsicum species from Southeast Asia. Twelve major protein bands were resolved by SDS-PAGE, nine of which were monomorphic. Of the polymorphic bands. Band 3 was the most common, occurring in 180% of the accessions. Band 7 was the least common band occurring only in 15 of the 100 accessions used in the study. Six banding patterns (BP) were exhibited by the three Capsicum species. BP 4 was the most common, exhibited by 63% of the total population. BP 1 was found to be least common, occurring in only 1 accession (1% of total population). BP I was found to be specific to C. chinense Jacq., while BP 6, 7 and 13 were specific to C annuum L No band pattern was observed to be specific to C..frutescens L. Frequency distribution, yielded the geographic distribution of specific bands and band patterns. Specific protein bands and band patterns predominated in the countries of origin. Band 1 predominated in Indonesia, Band 3 in the Philippines and Band 7 in Thailand. BP 1 and BP 7 predominated in the Philippines. BP 4 in Thailand and Indonesia, BP 5 and BP 13 in Thailand and BP 6 in Indonesia. Only BP 13 was not exhibited by the 17 accessions from the Philippines. Hence, the Philippines has the greatest diversity of banding patterns among the four Southeast Asian countries. Similarity index (SI) values showed that interspecific variation is greater than intraspecific variation. Among the three species, C. annuum L. was observed to exhibit the highest intraspecific variation (93.2), next is C frutescens L. (96.7), while C. chinense Jacq. has the lowest intraspecific variation (100.0). Based on the SI values and average linkage dendrogram, was found to be most closely related to (7. frutescens L., while C. chinense Jacq. is least closely related to C. annuum L. and C. frutescens L. Thus, the SDS-PAGE protein profile was able to determine the genetic diversity and divergence of the three Capsicum species.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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