A preliminary study on the effects of Benlate (Methyl-1- (Butylcarbamoyl)-2 Benzimidazolectarbamate) on the growth and cellulolytic activity of Trichoderma pseudokoningii, RIFAI1

Date

4-1985

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Virginia C. Cuevas

Abstract

Studies are needed to determine the possible effects of pesticides on non-target organisms. This work investigates the effect of t%eth1-1-(Butylcarbamoy1)-2-klienzymidazolecar3amate (Benlata) a fungicide on the growth, spore germination and cellulolytic activity of Trichoderma pseudokoningii Rifai. The test organism was cultured in Leisola and Kauppiner's, culture medium with cellulose powder as sole source of carbon, and in rice straw. Benlate was added at the recommended dose of 375 ppm and in decreasing concentrations of up to 1 ppm simultaneous with the inoculation of the organism to the medium and 10 days after inoculation. Growth in the culture medium was measured by harvesting the mycelia and getting the dry weight. The cellulase assay by Mandel was used to measure cellulolytic activity colorinetrically. Effects of the addition of Benlate on the culture medium was monitored every 2 days after addition of the fungicide up to the 22nd day. Effects of Benlate on decomposition of rice straw wan determined by adding the fungicide 15 days after inoculation of the fungus to the rice straw. Dry weight of rice straw an obtained on the 15th and 30th day. The effect of the fungicide was measured by comparing the rice straw dry weight before addition of the fungicide and 15 days after. When different concentrations of Benlate was added at the time of inoculation of the organism to the medium, no spore germination and hence no growth occurred. Thin suggests the.t even at 1 ppm concentration, the fungicide with effective in inhibiting spore germination of the organism. Growth of the organism was severely inhibited after Benlate was added on the tenth day at concentrations of 375 ppm and 1133 ppm which are the recommended doses. At lower concentrations (frog 1-94 ppm) the fungicide did not exert significant effects on the organised mycelial development. 14owever, cellulase activity was significantly reduced at all concentrations of Benlate added to the medium. Results obtained on the effect of Benlate on the rate of rice straw decomposition inoculated with T. pseudekoringii indicate that the fungicide has significant inhibitory effect at concentrations ranging from 25 ppm up to recommended dose of 375 ppm. The fungicide has no significant effects on the decom position process only at concentration of ppm.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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