Utilization of algae in bioremediation of abattoir wastewater under laboratory conditions

Date

6-2000

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Macrina T. Zafaralla

Abstract

RONALD DENNIS V. MACATBAG. Utilization of Algae in Bioremediation of Abattoir Wastewater under Laboratory Conditions. University of the Philippines at Los Banos, Laguna.

Adviser: Macrina T. Zafaralla

Raw abattoir effluent from Putatan, Muntinlupa city had the following qualitative and quantitative characteristics: grayish black in color with a strong hog manure odor and slightly acidic at pH 6.63; it had a temperature of 30 °C and mean optical density of 0.928. It had a salinity of 0.02 ppt resulting to a high electrical conductance (EC) (2100 umhos). The dissolved oxygen (DO) was low at 0.4 mg/L. The total alkalinity had a mean of 36 ppm while the total hardness was 460 ppm. Ammonia-N and ortho-P levels were high at 2190 ug/ L and 147 ug/ L, respectively. Sterilization result to: increase in DO content, raise to pH 9; decrease concentrations of the parameters given above. Using the sterilized abattoir effluent, culture media in varying concentrations were prepared as follows: 100% (M 1), 75% (M2), 50% (M3), and 25% (M4). The chemical characteristics of each of the media were compared. Ammonia-N did not exhibit any trend in concentration among the media unlike ortho-P, which gradually declined with dilution. Two treatments were prepared: a) uni-algal (Chlorella vulgaris); and b) algal mix (C. vulgaris, Scenedesmus quadricauda and Botrydiopsis sp.). Both the uni-algal and algal mix treatments exhibited the fastest growth in the M4 medium. C vulgaris dominated in all media given the algal mix. Botrydiop.sis sp. disappeared in all media after three days of culture. The ability of algae to cleanse the abattoir effluent when used in the form of uni-algal and algal-mix inocula was determined. Maximum diminution in the concentrations of total alkalinity and ammonia-N were observed in the MI medium. The highest percentage decline in ortho-P was observed in the M4 medium when both the uni-algal and the algal mix inocula were used. Between the uni-algal and the algal mix treatments, the latter was superior in terms of ortho-P and ammonia-N removal from the wastewater. However, ammonia-N reduction was not solely attributed to algal consumption.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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