Isozyme analysis of selected table-type banana (Musa spp.) cultivars in the Philippines

Date

4-2002

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Co-adviser

Felipe S. Dela Cruz

Abstract

Madrinan, Sharon 1.. March 2002. University of the Philippines Los Banos. Isozyme Analysis Of Selected Table-Type Banana (Musa Spp.) Cultivars In The Philippines.

Adviser. Dr. Merlyn S. Mendioro, Institute of Biological Sciences (IBS) Co-adviser : Dr. Felipe S. dela Cruz Jr., Institute of Plant Breeding (IPB)

Isozyme analysis of five selected table-type banana cultivars, namely: Kinalamay, Latundan Puti, Manang Costa, Mangipod, and Manifun was conducted through starch gel electrophoresis. Four enzyme systems, namely malate dehydrogenase (MDH), 6-phosphogluconate dehydrogenase (PGD), phosphoglucoisomerase (PG1), and phosphoglucomutase (PGM), were used to detect isoyme polymorphism in 21 varieties of table-type bananas. Two areas or loci of activity were observed for each enzyme used. MDH has a total of 21 bands with which 19 band patterns were formed, classifying 4 of the 5 unknown cultivars. PGD has 12 bands and 11 band patterns that were used to classify all of the unknowns. PGI has 26 bands and 18 band patterns, which was able to classify 4 of the 5 unknowns. PGM, which has 10 bands and 10 band patterns was only able to classify 2 of the 5 unknowns. The five unknowns were assigned their probable genomes. Kinalamay, Latundan Puti and Manang Costa were assigned as AAB, while Mangipod and Manifun were assigned as AAA. Dendrograms that were established using the Numerical Taxonomy and Multivariate Analysis System (NTSYS) program showed the relationships between and among the banana cultivars and this is concordant with the genomes assigned to the 5 selected table-type banana cultivars.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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