Induction of mutation in periwinkle, Catharanthus roseus (L.) don. using sodium azide

Date

10-1996

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Abstract

Maghirang, Reycel DM. 1996. University of the Philippines, Los Banos. INDUCTION OF MUTATION IN PERIWINKLE, CATHARANTHUS ROSEUS (L.) DON. USING SODIUM AZIDE.

Adviser: Dr. Merlyn S. Mendioro

ABSTRACT

To induce mutation in periwinkle, Catharanthus roseus (L.) Don., the seeds were soaked in different concentrations of sodium azide dissolved in 0.1 M phosphate buffer (pH 3). The effects of sodium azide on seed germination, plant morphology, meiosis and pollen fertility were studied. Sodium azide was capable of inducing seed germination delay. Varietal difference with regards to susceptibility to azide-induced delay in germination was evident between the pink and white varieties, such that the white variety was more sensitive. Furthermore, the seed viability of the white variety was negatively affected. Sodium azide caused plant height increase in pink variety while it caused slower growth in white variety. Several morphological abnormalities were present in the leaves, especially in plants exhibiting severe stunted growth, such as mottling, irregular leaf-shape, irregular leaf-arrangement, yellowing, white spots and necrotic tips. The percentage of plant that flowered decreased with increasing azide concentration. The minor increase in the abnormal meiotic configuration may have resulted to the decrease in pollen fertility.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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