Morphological and cytological characterization of Moringa collection of the Asian Vegetable Research and Development Center, Taiwan

Date

4-2004

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Genaleen Q. Diaz

Abstract

MALLILLIN, JESSICA PAULA C. University of the Philippines, Los Banos. April 2004. Morphological and Cytological Characterization of the Moringa Collection of the Asian Vegetable Research and Development Center, Taiwan. US Biology (Genetics). Advisers: Ma. Genaleen Q. Diaz, Ph. D. and Liwayway M. Engle Ph. D. ABSTRACT Morpho-agronomic and cytological characteristics were observed in different Moringa species. M. oleifera, M. drouhardii, stenopetala, and M. peregrina showed distinct morphological characteristics. M oleifera has pistil longer than stamens. M. peregrina is characterized by small leaves, leaflets, more secondary rachis, and distinctive pink flowers. M drouhardii is the only species with tall growth habit. A cluster analysis of the quantitative characters showed that M drouhardii had the highest dissimilarity value (39.332) separating it from the other accessions. M. peregrina was closely related to M oleifera ineludinp, them in one cluster. Unclassified accessions were identified based on the species they clustered with. Iron acetocarmine squash technique was used to determine the chromosome number and meiotic behavior of Al. oleifera and Al. peregrina. Both have chromosome number of 2n=28 though a high degree of quadrivalent formation was observed. Chromosome behavior in M oleifera showed the occurrence of low frequencies of normal cells at metaphase 1 and anaphase I (48.7% and 59.8%, respectively). However high frequencies of normal cells were observed at telophase I (94.7%). In M. peregrina, a relatively high number of normal metaphase I cells was observed but exhibited lower frequencies of normal cells at anaphase I and telophase 1 (68.7% and 60.9%, respectively). Abnormalities exhibited like laggards and bridges were noted in both species. Pollen fertility of M oleifera, peregrina and M. slenopetala. was determined using 12KI staining. Highest pollen fertility was observed at 64.19% for M. oleifera, 57.51% for M. peregrina and the lowest fertility was M. slenopelala with 56.2%. The relatively low pollen fertilities of M. oleifera and M. peregrina could be attributed to the occurrence of univalents, tetravalents and chromosome abnormalities in the different meiotic stages.

Location

UPLB Main Library Special Collections Section (USCS)

Document Type

Thesis

This document is currently not available here.

Share

COinS