Putative isoforms of lysophosphatidic acid acyl transferase gene in coconut (Cocos nucifera L.)

Date

10-2003

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Rita P. Laude

Abstract

MANZO, MICHELLE TOTANES. 2003. PUTATIVE ISOFORMS OF LYSOPHOSPHATIDIC ACID ACYL TRANSFERASE GENE IN COCONUT (Cocos Nucifera L.). University of the Philippines Los Banos. B.S. Biology major in Genetics. Major Adviser: Dr. Rita P. Laude Co-adviser: Prof. Marni E. Cueno

ABSTRACT

The putative isoforms of lysophosphatidic acid acyl transferase (LPAAT) gene in coconut (Cocos nucifera L.) were detected using the 3' rapid amplification of cDNA ends (3'RACE). The cDNAs of 4-, 5-, and 6-month-old coconut endosperm were quantified with an estimated concentration of 250ng/A. In the initial protocol, using combined hot-start and touchdown PCR with annealing temperature of 54°C, no electrophoretic bands were produced. Optimization was done using, cold-start PCR with annealing temperature of 54°C and combined hot-start and touchdown PCR with varying annealing temperature (59-49°C), but still no bands were observed. However, with combined hot-start and touchdown PCR, using a lower annealing temperature of 42°C, two bands were detected (700 by and 400 bp) at the 6-month-old coconut endosperm. Absence of bands was observed at 4- and 5-month-old coconut endosperm. The results suggested that LPAAT gene is actively transcribed at the 6-month old coconut endosperm while it is not yet expressed at 4- and 5-month old coconut endosperm, thus, indicating that LPAAT gene is ontologitally expressed.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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