Stomatal density and meaurement seedlessness in colochine-treated calamansi [ x Citrofortunella microcarpa (Bunge) wijnands]

Date

6-1994

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Violeta N. Villegas

Co-adviser

Reynold B. Pimentel

Abstract

The effect of colchicine on stomatal density, stomatal measurement and degree of seedlessness in calamansi plants was investigated. These parameters are useful in the preliminary survey of possible polyploids. Lateral buds of calamansi plants were treated with 0.1 % colchicine for 2 hrs and were shoot-tip grafted on calamandarin rootstocks. Results obtained on stomatal density revealed that nine plants (CA 1, CA 6, CA 26, CA 3, CA 27, CA 8, CA 17, CA 20 and CA 31) had the least number of stomates. On stomatal measurement, eleven plants (CA 1, CA 8, CA 31, CA 20, CA 3, CA 27, CA 6, CA 26, CA 33, CA 17 and CA 21) had the highest stomatal measurement. The evaluation of degree of seedlessness showed that six plants (CA 23, CA 27, CA 26, CA 31, CA 15 and CA 8) contained more aborted seeds than viable seeds. CA 1, CA 24, CA 20, CA 18 and CA 2, exhibited low fruit setting as compared to the untreated plants. Putting the data together, it is hypothesized that CA 27, CA 1, CA 26, CA 31, and CA 8 are putative polyploids because they have lesser number of stomates, have bigger stomatal measurement and their fruits contain more aborted seeds. Since these parameters are not absolute indicators of ploidy levels in plants, a cytological examination is necessary.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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