Genetic variability in accessions of Oryza meyeriana Zoll. et Mor. ex Steud and O. granulata Nees. et Arn. ex Watt. detected with morphological and microsatellite markers.

Date

4-2010

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Neilyn O. Villa

Abstract

The genetic diversity of 77 plants representing seven accessions of Oryza granulata and nine accessions of 0. meyeriana, being vegetatively propagated in the International Rice Genebank at IRRI were analyzed using morphological and molecular markers. The plants were scored using 22 qualitative and 12 quantitative traits and 8 SSR markers. Awn presence, stigma color and anther color, and panicle axis were invariant within the Oryza meyeriana complex. Cluster analysis based on both qualitative and quantitative characters showed the formation of distinct clusters for 0. granulata and 0. meyeriana, although a few representative plants deviated from its expected grouping. Principal Components Analysis of the quantitative traits showed that spikelet length and sterile lemma length are important characters in distinguishing the two species. Among the molecular markers, the PIC value was highest at 0.6859 (RM 224). 0. granulata showed a total of 21 alleles compared to 16 in 0. meyeriana. Cluster analysis based on 23 alleles showed the tendency of 0. granulata and 0. meyeriana to form separate clusters. Results from both morphological and molecular markers showed two 0. meyeriana plants, 104989_M4 and 104989_M5 to cluster with 0. granulata, suggesting possible mixture. This event impacts on the vegetative conservation of wild species, indicating the need for measures to prevent cross pollination or a risk management procedure that precludes human error in vegetative propagation.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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