Karyotype analysis of in vitro cultured human leukocytes treated with banaba (Lagerstroemia speciosa L.) leaf decoction

Date

4-2001

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Abstract

Cells from Giemsa-stained slides of male human leukocytes cultured in vitro treated with different concentrations (0%, 1%, and 3%) of banaba (Lagerstroemia speciosa L.) leaf decoction were examined. The chromosomes were arranged in a karyotype and their relative lengths were calculated. Results showed that although the treatments with banaba leaf decoction caused changes in the chromosome length. it did not significantly change the relative lengths of the chromosomes. A correlation was done between the slope and the change in length of the chromosomes. Chromosomes 3, 8, 13, 20, and X chromosome exhibited continuous decrease in length as the concentrations of banaba leaf decoction increased. Chromosome 3 appeared to he the most condensed since it exhibited the most negative slope (-0.236). Chromosomes, which showed a continuous increase in lengths as the concentration increased, were chromosomes 1, 6, 7,10, 15,19, and 22. Chromosome 1 has the most positive slope (0.315). the rest of the chromosomes manifested a fluctuating reaction to the banaba leaf decoction. Group B chromosomes had the most positive slope (0.108) while Group D had the most negative slope (-0.087). The results imply that the change in the length of the chromosomes could not be attributed to the banaba leaf decoction treatment.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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