Genetic diversity based on morphometric analysis and protein profile using SDS-page in two populations of the giant honeybee (Apis dorsata F.) in the Philippines

Anna E. Merca

Date

3-1999

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Rita P. Laude

Abstract

Merca, Anna E. April 1999. University of the Philippines at Los Banos. GENETIC DIVERSITY BASED ON MORPHOMETRIC ANALYSIS AND PROTEIN PROFILE USING SDS-PAGE IN TWO POPULATIONS OF THE GIANT HONEYBEE (Apis dorsata Fab.) IN THE PHILIPPINES.

Adviser: Dr. Rita P. Laude

SDS-PAGE and morphometric analysis was done to determine the diversity between two populations of the giant honeybee from Jubileeville, Bay, Laguna and Forestry Campus, UPLB, College, Laguna, Philippines. A total of five protein bands of high molecular weight were present in both populations. Bands 1, 2, and 3 were 100 % present in both populations. Band 4 was 100 % present in the Jubileeville bee population while it was only 85 % present in the Forestry bee populations . Band 5 had the lowest % occurrence of 20 and 40 % for the Jubileeville and Forestry giant honeybee populations, respectively. A total of three protein band patterns (BP) were observed. These were BP-A, with bands 1, 2, 3, 4, and 5; BP-B with bands 1, 2, 3, and 4; and BP-C with bands 1, 2, and 3. BP-A and BP-B was common to the two populations while BP-C was observed only in the Forestry population. The Jubileeville population had a high similarity index (SI) of 80-100% while the Forestry sample gave a SI of 60-100%. The two populations exhibited an average SI of 71%. Morphological measurements showed that the two populations were separate from each other by clustering into two separate groups based on location. The only body part that can possibly distinguish the two populations from each other was the distance of the wax mirror. It may be concluded that the populations are highly similar to each other both morphologically and biochemically based on protein composition.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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