"Association of allelic diversity in lycopene B-cyclase gene with lycop" by Pauline Andrea M. Muyco

Association of allelic diversity in lycopene B-cyclase gene with lycopene and B-carotene levels and antioxidant activity in tomato (Solanum lycopersicon L.)

Date

4-2008

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Abstract

MUYCO, PAULINE ANDREA M. 2008. Association of Allelic Diversity in Lycopene II-Cyclase Gene with Variation in Lycopene and B-carotene Levels and Antioxidant Activity in Tomato (Solanunt lycopersicon, L.) University of the Philippines Los Banos, B.S. Biology Major in Genetics

Adviser: Dr. Merlyn S. Mendioro (UPLB) Co-adviser: Dr. Robert dela Pella (AVRDC)

ABSTRACT Population structure of 48 Solanum lycopersicon L. lines from the Asian Vegetable Research and Development Center's (AVRDC) core tomato collection was evaluated using ten polymorphic SSR markers. Gene diversity value was 0.67, indicating a moderate level of genetic diversity in the population. Lycopene 13-cyclase (Cyc-fi) gene of 12 tomato lines having the highest and lowest lycopene and beta-carotene levels, two Tomato Yellow Leaf Curl Virus (TYLCV) resistant lines, and a heat tolerant line was sequenced and aligned using the BioEdit sequence alignment program. Allelic diversity of the gene was computed using DnaSP ver 4.20 and was found to be low at 0.00234. Entries 97L63 (B), CLN1314G (B) and FLA456 have single nucleotide polymorphisms (SNPs). Twenty SNPs were identified — one in 97L63 (B), ten in CLN1314G (B), and nine in FLA456. A tree showing the genetic relationships of the 15 lines based on Cyc-11 revealed two clusters — the orange-fruited cluster (97L63 (B), CLN1314G (B), and FLA456) and the red-fruited cluster (PI 129128, PI 138618, Nagcarlan, FMTT33, NCEBR-6 (o(), T5019 (dg,4), LA2996A (Del), LA2999 (g/). LA2997 (r), LA2644 (sh), FLA456, CLN1621L, and CLN2123A). Association of allelic diversity in Cyc-fi gene with variation in lycopene and (3-carotene levels and antioxidant activity in tomato was established using HelixTree SNP and Variation Suite program. HelixTree identified SNP 491 to be significantly associated with lycopene and 13-carotene levels and antioxidant activity. The strongest association was between SNP 491 and 13-carotene. SNP 491 could be a potential marker for selection of varieties having high 13-carotene content. Identification of alleles or SNPs that increase carotenoid levels and compositions will assist breeders in developing tomato varieties with enhanced nutritional properties.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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