Callus induction in stem base and leaf tissues of shallot (Allium ascalonicum L.)

Date

4-1991

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Teresita Lantin-Rosario

Abstract

The appropriate sterilization procedure and the requirements for callus induction of shallot. (Allium ascalonicum L.) were investigated to develop technique for rapid propagation. Stem base and leaf tissues derived from shallot bulbs were sterilized using different techniques. Low percent contamination was obtained resulting to a high percent survival when both explants were washed with detergent and water for 7 minutes, then soaked in 70% ethyl alcohol for 3 minutes before soaking in 50% zonrox (commercial bleach) for 15 minutes. The explants were not rinsed with sterile distilled water after the zonrox was removed. The stem base and leaf tissues were inoculated on 25 media treatments with combined concentrations of B5 nutrient medium, sugar (20 grams/liter), indoleacetic acid (IAA), 2, 4-chlorophenoxyacetic acid (2.4-D), benzyladenine (BA), naphthaleneacetic acid (NAA) and kinetin (KIN), More callus was induced at 5mg/L 2,4-D with or without 5 mg/L KIN.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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