RAPD analysis for the detection and evaluation of genetic polymorphism in selected abaca (Musa textilis Nee) germplasm in the Philippines

Date

4-1995

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Desiree M. Hautea

Abstract

NATURAL, TRICIA MARI-LEN P. University of the Philippines Los Banos. April, 1995. RAPD Analysis for the Detection and Evaluation of Genetic Polymorphism in Selected Abaca (Musa textilis Nee) Germplasm in the Philippines. Adviser: Dr. Desiree M. Hautea The technique of randomly amplified polymorphic DNA (RAPD) was used to investigate DNA sequence variation in eight clones of abaca (Musa textilis) obtained from existing germplasm collection. Conditions were optimized for the generation of RAPD markers for abaca. A total of 100 different 10-mer random primers from Operon Technologies were tested to reveal DNA polymorphisms among the different clones. Fifty eight out of the 100 random primers tested yielded intense and well-resolved amplification products. Four primers (OPU-01, OPV-14, OPV-20 and OPX-04) used together enabled the discrimination of all abaca genotypes tested. However, primers OPU-01 and OPX-04 proved sufficient in discriminating genotypes collected from the Bicol region with collections from other places. This stud shows that RAPD analysis can be employed to generate genet fingerprints of eight abaca genotypes and estimate the genetic relationships based on the analysis of polymorphism identified.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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