Genetic diversity based on morphology, simple sequence repeats (SSR) and conserved ortholog set II (COSII) markers and crossability relationship in eggplant (Solanum melongena L.) and related species

Date

4-2009

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Neilyn P Ona-Villa

Abstract

NEPOMUCENO, ROBERT A. 2009. Genetic Diversity Based on Simple Sequence Repeats (SSR) and Conserved Ortholog Set II (COSH) Markers and Crossability Relationship in Eggplant (Solarium melongena L.) and Related Species. University of the Philippines, Los Banos. B.S. Biology major in Genetics Adviser: Prof Neilyn 0. Villa Co-Adviser: Dr. Robert dela Pena ABSTRACT Genetic diversity analysis was conducted on 48 eggplant accessions maintained at AVRDC gene bank. The study was based upon morphology, molecular markers and crossability relationship. Morphological characterization involved the use of 53 morphological descriptors. Cluster analysis revealed five species-specific groupings. Cluster I, joined by 82% similarity was composed of S. sisymbrifolium, S. indicum and S. ferox. Cluster II, 93% similarity was composed of S. melongena, S. aethiopicum, S. anguivi and S. viarum. Cluster III, IV and V were composed of S. torvum, S. scabrum and S. macrocarpon, respectively. Twenty-four primer pairs of SSR and 6 of COS II markers were used in the study. Cluster analysis failed to generate any species-specific groupings. This is primarily attributed to a small number of alleles generated. Clustering based on geographical origin was observed which may probably be due to improper species circumscriptions and gene flow. Crossability relationship study showed that the crosses between S. melongena, S. macrocarpon and S. aethiopicum and S. viarum had high fruit setting (highest was 36%). It can be stated then that AVRDC's eggplant germplasm collection represents very high genetic diversity and is a potential source of useful traits for crop improvement.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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