"Comparative karyotypes of three Philippine native orchids, Phalaenopsis mariae burb ex B. S. Wms, Phalaenopsis philippinensis golamco ex Fowlie and Tang and Phalaenopsis sanderiana RCHB F."

Date

4-2005

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Celia B. Dela Viña

Abstract

OBLENA, MARI ANNIELETTE SICAT. 2005. Comparative Karyotypes of Three Philippine Native Orchids, Phalaenopsis mariae Burb ex B. S. Wms., Phalaenopsis philippinensis Golamco ex Fowlie and Tang and Phalaenopsis sanderiana Rchb. f. University of the Philippines Los Banos. BS Biology Major in Genetics.

Major Adviser: Professor Celia B. Dela Villa

ABSTRACT Chromosome behavior during mitosis and chromosome number, relative length and type based on centromere position were determined in Phalaenopsis mariae Burb ex B.S. Wms., P. philippinensis Golamco ex Fowlie and Tang and P. sanderiana Rchb. f. Slides were prepared using the aceto-carmine squash technique. Cells exhibited normal mitotic activity in all the three species. The mitotic indices were relatively low; the highest obtained was from P. sanderiana (16.33%), followed by P. mariae (15.81%), then P. philippinensis (13.40%). Representative karyotypes constructed from prometaphase cells of the individual species revealed a diploid chromosome number of 2n = 38. The chromosomes were comparatively small, with average relative chromosome lengths ranging from 0.036-0.076 for P. philippinensis, 0.037-0.074 for P. mariae and 0.039-0.072 for P. sanderiana. All the chromosomes were metacentric in the three Phalaenopsis species.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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