Genetic diversity in seed proteins among five Solanum species from the Philippines using SDS-page

Neilyn P. Ona

Date

4-1996

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Dolores A. Ramirez

Co-adviser

Abella C. Dela Viña

Abstract

Ona, Neilyn P April 1996 University of the Philippines, Los I3ailos GENETIC DIVERSITY IN SEED PROTEINS AMONG FIVE SOLANUM SPECIES FROM THE PHILIPPINES USING SDS-PAGE.

Adviser Dr Dolores A Ramirez Co-adviser Dr Abella C dela Villa

This study was conducted to determine genetic differences among five non-tuberiferous Solarium species from the Philippines, namely S. melongena. S aethiopicum, , S. macrocarpon, S. nigrum, and S. surattense, using SDS-PAGE of seed proteins The objectives were to determine the SDS-PAGE seed protein hand patterns in the 220 accessions of the five Solarium species; to calculate the frequency of occurrence of specific bands within each species; and to infer the taxonomic relationship among the five Solarium species. A total of 19 major bands was resolved by SDS-PAGE. Twelve protein band patterns were exhibited by the Solarium accessions. Seven band patterns were observed within S. melongena indicating sufficient genetic diversity among the local eggplant collection These band patterns are BP 1 (composed of bands 2, 4, 7, 10. 12. 14, and 16). BP 2 (BP 1 plus band 3), BP 3 (BP 1 bands except band 2 but plus band 17), BP 4 (BP 1 bands plus band 17), BI' 5 (BP 1 bands except band 16), BP 17 (bands 2, 5, 6, 10, 12. 16. and 17), and BP 8 (BP 1 bands plus band 9). Except for BP 1, these band patterns were specific to S. melongena. Likewise, BP 6 was specific to S. aethiopicum, BP 9 to S. surattense, BP 10 and 11 to S. nigrum, and BP 12 to S. macrocarpon. Low intraspecific variation was observed among the wild species probably due to the small sample size and the use of accessions originating from neighboring provinces. The taxonomic relationship of the four wild Solarium species to S. melongena was inferred based on similarity index values and a single linkage dendogram obtained through cluster analysis S. melongena was found to be most closely related to S. aethioipicum, followed by S. macrocarpon, S. surattense. and S. nigrum. This result generally agrees with that of serological studies, chloroplast DNA analysis, iillozyme analysis, and crossability and hybridization experiments The wide genetic base of the non-tuberiferous Solarium determined by the SDS-PAGE seed protein profile generally corroborates known morphological data. Thus. SDS-PAGE may prove to be a more useful tool to characterize genetic diversity since it is less expensive, easier to implement and does not require special facilities

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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