"Hemocytes of two orb-weaving spiders, Neoscona nautica ( C.L. Koch ) and Neoscona puntigera (Doleschall) (Araneae: Araneidae)"

Aleli F. Onia

Date

10-1998

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Adelina A. Barrion

Abstract

ONIA, ALELI FLORES, College of Arts and Sciences, University of the Philippines Los Banos, College, Laguna. October, 1998. HEMOCYTES OF TWO ORB-WEAVING SPIDERS, Neoscona nautica (C.L. Koch) AND Neoscona puntigera (Doleschall) (ARANEAE: ARANEIDAE).

Major Adviser: DR. ADELINA A. BARRION

Different types of hemocytes present in the hemolymph from the legs of two orb-weaving spiders, Neoscona nautica (C.L. Koch) and Neoscona puntigera (Doleschall), were determined employing the squash technique, staining with Wright's stain and fixation through air-drying. Hemocyte types were identified using the nomenclature system of Gupta (1979). Differential and total hemocyte counts as well as the average sizes of each cell type were determined for each species. Hemolymphs of N. nautica and N. puntigera contained granulocytes (GR), plasmatocytes (PL), prohemocytes (PR), and spherulocytes (SP), with the addition of adipohemocytes (AD) in N. puntigera. In both species, the most dominant form was PR. In N. nautica, PRs (52% occurrence) were followed by PLs (26%), then GRs (19%), and finally SPs (3%). In N. puntigera, after PR (46%) the next dominant blood cell type was PL (21%), followed by GR (18%), then AD (13%) and lastly SP (2%). These four cell types present in the two spiders did not vary in terms of size, shape and frequency of occurrence. Statistical analysis revealed that there were no significant differences in the counts of GRs, PLs, PRs, and SPs between N. nautica and N. puntigera. Mean sizes of all common cell types in both species were also not significantly different. However, total hemocyte counts differed significantly between the two species, with N. puntigera (1231 blood cells) having a higher cell count than N. nautica (1123 cells).

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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