Isozyme analysis in eggplant, Solanum melongena L., S. linociera, and their F1 hybrids

Date

4-2005

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Abstract

ORDILLO, LUWALHATI SARA PADON. 2005. Isozyme analysis in eggplant, Solarium melongena linociera, and their F1 hybrids. University of the Philippines Los Banos. B.S. Biology major in Cell Biology.

Major Adviser: Dr. Merlyn S. Mendioro

ABSTRACT lsozyme analysis was done on eggplant, Solunum melongena, S. linociera and their F1 hybrid. Ten enzymes namely Acid phosphatase (ACP11), Alkaline phosphatase (ALP), Esterase (EST), Phophogluconate dehydrogenase (PGD), Isocitrate dehydrogenase (ICD), Phosphoglucoisomerase (PGI), Phosphoglucomutase (PGM), Malic Enzyme (ME), Shikimate dehydrogenase (SDH), Malate dehydrogenase (MDH) were used in this study. Thirteen loci were identified using the 10 enzyme sysytems. S melongena, S. linociera and their F1 hybrid showed similar genotypes for loci ACPH 1, EST 1, 1CD 1, PGI 2, PGM I, SDH1, SDH 2 and MDH I. S. melongena and S. linociera were observed to be homozygous (SS) for ACPH I, PGM 2, SDH 2 and MDII 1. Fl hybrids were homozygous (SS) for the said loci. On the other hand, both parentals were observed to be heterozygous (FS) for EST 1, ICD 1, PGI 2, and SDH 1. The F1 hybrids produced were heterozygous as well. For ALP 1, S. melongena exhibited genotype SS, while S. linociera exhibited FS genotype. Hybrids produced from this cross exhibited FS genotype. S. melongena was observed to be homozygous FF for PGD 1, while S. linociera exhibited the FS genotype. The hybrid was observed to be heterozygous FS. For PGM 1, homozygous FF and SS genotypes were observed in S. melongena and S. linociera, respectively. A heterozygous genotype (FS) was observed in the F1 hybrid. The isozyme profile of the F1 hybrid showed inheritance of the alleles present in the parentals, S. melongena and S. linociera. Thus, hybrid nature was proven.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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