Genetic divesity of cherry tomato, Solanum lycopersicum var. cerasiforme L., accessions and other wild species in AVRDC - The World Vegetable Center using conserved Ortholog set II and Simple Sequence Repeat markers.

Date

4-2010

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Maria Cecilia S. Reamillo

Abstract

PALANCA, JOSEMIAE MUTYA F. 2010 Genetic Diversity of Cherry Tomato, Solanum lycopersicum var. cerasiforme L., and Other Wild Species in AVRDC-The World Vegetable Center Using Conserved Ortholog Set II and Simple Sequence Repeat Markers. University of the Philippines, Los Banos. B.S. Biology Major in Genetics

Adviser: Prof. Maria Cecilia S. Reamillo Co-adviser: Dr. Kadirvel Palchamy

ABSTRACT Using 24 Conserved Ortholog Set 11 and 105 Simple Sequence Repeat markers, 93 Solanum lycopersicum var. cerasiforme L. accessions, together with some wild species (S. pimpinellifolium, S. chilense, S pennellii, and S. hirsutum), in the AVRDC—The World Vegetable Center was analyzed for their genetic diversity. SSR markers were found to be more polymorphic compared to the COS 11 markers. A total of 581 alleles were generated with an average of 5.75 alleles per marker. Of these alleles generated, 303 -alleles (52.15%) were considered rare. Polymorphism Information Content (PIC) values ranged from 0 to 0.8544 with a mean of 0.3650. Shannon—Weaver Diversity index al') values ranged from 0 to 0.8827 with a mean of 0.4328. Using Jaccard's similarity coefficient, accessions were clustered in the UPGMA program of the NTSYS software. Dendrogram showed that the S. lycopersicum var. cerasiforme clustered with the cultivated tomatoes. Furthermore, accessions from similar locations tend to group together. Results showed that there is considerable genetic diversity among the cherry tomato accessions maintained in the AVRDC--GRSU gene bank.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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