Cytogenetic effects of ampalaya (Momordica charantia L.) tablet on human leukocytes cultured in vitro

Date

3-1999

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Abstract

PALMA, MARITES C. University of the Philippines Los Banos. March 1999. Cytogenetic Effects of Ampalaya (Momordica charantia L) Tablet on Human Leukocytes Cultured in vitro. Undergraduate Thesis. B.S. Biology (Cell Biology).

ADVISER: Mendioro, M.S., Ph. D.

To test the cytogenetic effects of ampalaya tablet on human leukocytes cultured in vitro, 4 to 5 drops of blood from seven male donors of ages 18-21 were incubated at 37 °C for 48 hours. They were then treated with four different concentrations of the tablet (0, 1, 3, and 5%) and cultured again for another 24 hrs. After which the cells were then harvested and cytologically observed. Results showed no significant difference in the mitotic indices between the control and treated groups. Mean mitotic indices of 0.130, 0.123, 0.113 and 0.109 were observed in the control, 1, 3, and 5% respectively. The treatment did not also show any significant differences on the tests for chromosomal gaps, breaks, and loose sister chromatids. However, the tablet significantly increased the occurrence of condensed chromosomes (control = 0.086; 1% = 0.086; 3% = 0.206; 5% = 0.157). Results imply that although the drug was not able to alter normal mitotic frequencies and induce chromosomal aberrations, the significant increase in chromosome condensation could be an abnormality which can possibly lead to abnormal cell function.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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