Genetic diversity in bitter gourd, Momordica charantia Linn. (Cucurbitaceae) based on SDS-page of leaf and seed proteins

Date

3-2000

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Adelina A. Barrion

Co-adviser

Liwayway M. Engle

Abstract

PANINGBATAN, ANDY TEODORO KWAN. College of Arts and Sciences, University of the Philippines, Los Banos. March 2000 Genetic Diversity in Bitter Gourd, Momordica charantia Linn. (Cucurbitaceae) Based on SDS-PAGE of Leaf and Seed Proteins.

Adviser: Dr. Adelina A. Barrion. Co-adviser: Dr. Liwayway M. Engle

Sodium dodecyl sulphate — polyacrylamide gel electrophoresis (SDS-PAGE) was performed in order to obtained the leaf and seed protein band patterns of the different Momordica accessions conserved at the Fengshan Tropical Horticultural Experiment Station of the Taiwan Agricultural Research Institute (TARI) and the Genetic Resources and Seed Unit (GRSU) of the Asian Vegetable Research and Development Center (AVRDC) at Shanhua, Tainan, Taiwan. Electrophoretic data show that 20 and 21 bands composed the 8 and 4 band patterns acquired for Momordica leaf and seed proteins, respectively. Since the similarity values acquired for the Momordica accessions of AVRDC ranged from 80% to 95% (leaf proteins) and 85.71% to 95.24% (seed proteins), AVRDC's collection is therefore mainly composed of one species, Momordica charantia L. The exception was that accession TOT 4034 is Momordica cochinchinensis Loureiro. Results of the analysis of data using average linkage cluster analysis further affirm the conclusion. Seed and leaf protein band patterns of wild and cultivated M. charantia were also obtained, analyzed and compared. No significant difference between cultivated and wild M. charantia was observed and the dendogram produced did not separate the wild and cultivated Momordica charantia into distinct groups.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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