Association of allelic diversity in capsanthin-capsorubin synthase gene with variation in capsandthin concentrations and antioxidant levels in pepper (Capsicudm sp.)

Date

4-2008

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Abstract

Genetic relationships among 44 accessions of pepper (Capsicum sp.) from the Asian Vegetable Research and Development Center (AVRDC) collection was established using polymorphic conserved orthologous set II (COS II) and simple sequence repeat (SSR) markers. Moderate population diversity was measured at a value of 0.71. CCS gene of the 22 pepper accessions with varying capsanthin concentrations was sequenced and aligned using BioEdit sequence alignment program. Allelic diversity of the gene was measured to be low at 0.00439 and was determined using DnaSP ver 4.20. Population structure of the sequenced genotypes resulted in two clusters with significant relationships according to levels of capsanthin concentration and taxonomic grouping. Single nucleotide polymorphisms (SNPs) were identified in 17 pepper accessions. Of the 28 SNPs, 18 were transition events while 10 were transversion. Association of the identified SNPs in capsanthin-capsorubin synthase (CCS) gene of 22 pepper genotypes and varying levels of capsanthin concentration was determined using Helix Tree SNP and Variation Suite. SNP 240 and 635 had been strongly associated with increased levels of capsanthin concentration and antioxidant activity. Both polymorphic sites obtained the most significant p value (p=0.30). The presence of strong association between two polymorphic sites with capsanthin and antioxidant levels could facilitate marker-assisted breeding of pepper with high capsanthin content and antioxidant levels that will contribute in improving human health.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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