Esterase isozymes of tilapia (Oreochromis niloticus Linnaeus) collected from two different lakewater sites

Date

10-1988

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ivan Marcelo A. Duka

Abstract

Electrophoretic analysis of esterase isozymes of 72 two and a half month old tilapia collected from two different Lake water sites was done, 36 from Sucol, Calamba portion of Laguna de Bay (Site A) and 36 from Sampaloc Lake, San Pablo City, Laguna (Site B). Both sample sets exhibited multiple esterase isozymes in complex and heterogenous patterns and all bands exhibited varying intensities ranging from low to high. A total of 14 bands were obtained from the site A sample, each sample with three to nine bands. The fastest moving band, isozyme 2, had an Rf value of 0.934 and the slowest moving hand, isozyme 16, had an Rf value of 0.155. Isozymes more grouped into four zones with zone 4 being uniquely observed in this sample net. A total of 12 bands were obtained from the site B samples, each sample with three to eight bands. The most anodal band, isozyme 1, had an Rf value of 0.985 and the slowest moving band isozyme 12, had an Rf value of 0.468. Isozyme were grouped into three zones. Thus, both population sets exhibited heterogeneity of banding patterns, however, site B samples showed a less heterogenous than those from site A. The variation in banding patterns of the two populations may be attributed to differences in their environmental conditions. The more stable the habitat (i.e. Sampaloc Lake), the less heterogenous the esterase isozymes banding pattern and in unstable habitat (i.e. Sucol, Calamba portion of Laguna de Bay) The esterase isozyme banding pattern was more heterogenous and complicated.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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