Acute Dermal Toxicity Test of Plasma Assited Chitosan-Acrylic Acid Hydrogel Dressing in Rabbits

Date

2024

Degree

Doctor of Veterinary Medicine

Adviser/Committee Chair

Melissa Marie R. Rondina

Co-adviser

Maria Amelita C. Estacio

Abstract

An acute dermal toxicity test was conducted following the Organization for Economic Cooperation and Development (OECD) Guideline 404 for the new locally produced plasma-treated chitosan acrylic acid (Cs-AA) hydrogel dressing using New Zealand white (NZW) rabbits. The plasma-treated Cs-AA hydrogel and control dressing was applied on the right and left shaved dorsum of each rabbit, respectively and occluded with sterile gauze and non-irritating medical tape for 24 hours. Cage side observations for skin abnormalities like erythema and edema, and systemic signs of toxicity, morbidity, and mortality were done for 14 days. Then, the rabbits were anesthetized, and an elliptical skin biopsy was performed for histopathologic examination. The results show that application of plasma-treated Cs-AA hydrogel dressing did not elicit skin reactions like erythema, and edema in rabbits. No overt signs of toxicity like salivation, diarrhea, tremors, lethargy cardiopulmonary depression, neurologic abnormalities, or behavioral changes were observed. Feed and water intake, body weight, and body weight gain were not affected. Histopathologic examination revealed comparable normal appearance and histologic features of skin of treated control dressing and plasma-treated Cs-AA hydrogel dressing. There were no signs of epidermal or dermal thickness, erythema, edema, inflammatory cell infiltration, acanthosis, and hyalinization. These findings suggest that the plasma-treated Cs-AA hydrogel dressing is non-toxic and safe as a potential topical biomedical product for wound management in animals and humans.

Language

English

Location

UPLB College of Veterinary Medicine (CVM)

Notes

Awards: PALAS Best Undergraduate Thesis Award on Laboratory Animals

Document Type

Thesis

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