Cytogenetic effects of sambong (Blumea balsamifera L.) tablets on human leukocytes in vitro

Date

3-1998

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Mendioro M. S.

Abstract

Sambong (Blumea balsamifera L) is one of the medicinal plants in the country that is now being processed into a promising diuretic drug. Three concentrations of this tablet (0.5, 1.0 and 1.5%) were used to test the cytogenetic effect of the herbal tablet to human leukocytes in vitro. Two randomly selected male students act as blood donors. The blood was cultured in a chromosome medium for 48 hours, after which the treatment were added to the vial and cultured Again for another 24 hours. The cells were then harvested and mounted to a slide for analysts. Analysis of Variance was used to test if there is 4 significant cytogenetic effect . Results showed no significant difference in the mitotic indexes between the three treatments. Mean mitotic index of 0.12509 (0.5%), 0.11139 (1.0%) and 0.10409 (1.5%) did not give any significant increasing or decreasing trend. For gaps and breaks, no significant difference was observed between the treatment. The observed frequencies - 0.0199 (0.5%), 0.0441 (1.0%), 0.0399 (1.5%) for gaps and 0.0200, 0.0147, 0.0399 respectively for breaks - are very low to be able to establish the chromosome breaking potential of the tablet. No significant difference was observed in loose sister chromatid. Very low frequencies were also observed ( 0.0448 - 0.5%, 0.0441 - 1.0%, and 0.0746 - 1.5%). The number of cells with condensed chromosomes have the same findings as that of the gaps and breaks.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

This document is currently not available here.

Share

COinS