Effects of glucose feeding and seed priming on submergence tolerance of rice (Oryza sativa L.) during germination

Date

4-2008

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Maribel L. Dionisio-Sese

Co-adviser

Abdelbagi M. Ismail

Abstract

Selected genotypes of rice (Oryza saint, L) exhibiting contrasting tolerance to submergence during germination, namely tolerant Khaiyan and Khao hlan on and intolerant IR42 and FR13A, were used to determine the effects of glucose feeding and seed priming on submergence tolerance. Percentage gemination and coleoptile and/or root growth were measured after seven days of germination whereas activities of alcohol dehydrogenase (ADM and pyruvate decarboxylase (PDC) were measured at different time intervals. Results showed that glucose feeding did not improve percentage germination and coleoptile growth of the four genotypes under hypoxia in the dark. Application of light, however. increased the percentage gemination and coleoptile growth of 1R42 under 20 and 10 o.t glucose addition. respectively, but not for Khaiyan. Enzyme analysis of germinated seeds supplied with 10 mM glucose revealed light-stimulation of PDC activity in both genotypes after 72 hours while ADH activity was not enhanced by light. No significant improvement on percentage gemination was observed with seed priming. However. primed Khao hlan on and IR42 showed improvement in coleoptile growth during hypoxia while FR13A did not respond. Moreover. the roots of Khao hlan on were reduced by priming while for intolerant 1R42 no significant reduction was observed. ADH and PDC activities increased from 24 to 72 hours by seed priming in Khao hlan on and IR42 but not in FR I3A. Results suggest that glucose feeding in light and seed priming enhanced coleoptile growth during hypoxia As increase in POE activity. which is a control point for anaerobic energy production, thus improving submergence tolerance of rice during germination.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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