Comparative electrophoretic analysis of some isozymes from selected organs of milkfish (Chanos chanos Forskal) collected from two separate areas of Laguna Lake: Los Baños and San Pedro

Date

10-1999

Degree

Bachelor of Science in Biology

Major Course

Major in Ecology Genetics

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ivan Marcelo A. Duka

Abstract

Starch gel eletrophoresis was used to determine bands of four enzymes namely: Alkaline Phosphatase (ALPH); Acid Phosphatase (ACPH); Esterase (EST) and Malic Dehydrogenase (MDH) in three organs (heart, muscle and liver) of milkfish collected from Los Banos and San Pedro, Laguna. Fisher's Least Significant Difference mode of statistical analysis showed no significant differences on man Relative Mobilities (RF) among ACPH, ALPH and MDH isozymes. This is true for site-organ, site-enzyme and organ-site interactions. Esterase isozymes however, showed significant variation only on organ-enzyme interaction. Therefore, despite the differences in mean Rf's seen among ACPH, ALPH and MDH isozymes from the same organ but different populations, their values are still statistically no significant. Similarity index (SI) of organ to organ comparison of isozyme bands for specific enzymes with population shows 100% SI values except for heart/liver and liver/heart organs which showed only 50% similarity index. Comparison of same organs for a specific isozymes from the two populations, however, shows 100% SI values, Therefore, isozymes bands for ACPH, ALPH and MDH are the same despite their differences in organ origin EST isozyme hands however, are organ specific EST isozyme bands of bean Is different from EST isozyme of cyanide muscle and liver Liver and epaxial muscle however. have the same isozyme bands Percent occurrence values of bands from same organs but different population show relatively close values except for ACPH isozymes ACPH Los Ratios and San Pedro heart occurrences are 96.67% and 90% respectively. ACPH muscle mock occurrences are 100% and 86.67% in Los Banos and San Pedro, respectively. ACPH Ines occurrency are 83.33% and 90% for Los Banos and San Pedro respectively ALPH bean occurrences are 100% in Los Banos and San Pedro. ALPH muscle occurrences are 100% and 96.67% in Los Ral1os and San Pedro. respectively. ALP11 liver occurrence is 100% both in Los Haf1os and San Pedro_ EST 2 heart occurrence is 96.67% both in Los 13shos and San Pedro PST 2 epaxial muscle occurrence is 100% both in Las Banos and San Pedro EST 2 liver occurrence is 100% both in LOS Ha. and San Pedro. EST 1 hurt occurrence is 73.33% both in Los Banos and San Pedro MITI bean occurrence is 100% both in Los Banos and San Pedro MINI epaxial muscle occurrences are 96.67% and 100% in Las 13311os and San Pedro. respectively Lastly. MD11 occurrence is 100% in both Los Banos and San Pedro. Comparison of organs within populations also shows close values except for isozyme of Los Banos Patent muscle of Los Banos ACPH are 96.67%, 100% and 83.33% for heart, muscle and liver respectively Percent occurrency in San Pedro ACPH arc 90%, 86_67% and 90% in hers, crams] muscle and liver, respectively. Los Banos ALPH occurrence is 100% for all organs observed Percent occurrences of San Pedro MPH arc 100%. 96.67% and 100% in helm epaxial muscle and liver, respectively Peron, occurrences of Los Banos and San Pedro EST 2 arc 96 67%. 100% and 100% in hear, epaxial muscle and him, respectively. Percent occurrence in Los Banos and San Pedro EST 1 is 73.33% for heart Percent occurrence of Los Banos MD11 are 100%. 96.67% and 100% for heart, epaxial muscle and liver. respectively Lastly. San Palm MD/1 occurrence is 100% in all organs This shows that isozyme bands mentioned arc major bands based on their occurrence values.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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