Physiological evaluation of elite salt tolerant genotypes of rice (Oryza sativa L.) at seedling stage and molecular characterization using microsatellite markers

Date

4-2006

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Co-adviser

Glenn B. Gregorio

Abstract

Three hundred eighty-one elite salt tolerant rice lines developed at The International Rice Research Institute were evaluated for seedling stage tolerance vis-a-vis ion uptake (Na+ and K+ in Simple Nutrient Additional Program (SNAP) culture solution at electrical conductivity (EC) of 12 dS/m. IR29 and 11266946-36-178-1-1 were used as sensitive and tolerant checks. respectively. In the screening for salinity tolerance at seedling stag, eight genotypes were found to be highly tolerant. 62 genotypes were tolerant. 170 genotypes were moderately tolerant, 121 salt-sensitive genotypes, and 23 genotypes were very sensitive. Using Atomic Absorption Spectrophotometer. all the lines were characterized for the uptake of Na. and K. and were classified according to the Ns.- K. uptake ratio range. The mean Na+--K+. ratios of all the genotypes in each Na+-K+. were compared with their mean screening scores. Correlation analysis indicated that at p=0.05. the association between the salinity tolerance score and Na+-K+ ion uptake ratio was significant. "the 181 elite salt tolerant lines were clustered based on combined physiological data and SES scores. The calculated minimum dissimilarity and maximum dissimilarity values were 3.84x10 and 2.795, respectively. The genotypes were characterized and clustered using 16 SSR 'makers. A minimum dissimilarity value of 8.33x10-2 was calculated while maximum dissimilarity value is 0.923. Upon comparison, both the genotypes with Na-K uptake ratio < 0.2 and > 1.0 were not entirely clustered together.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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