Biochemical changes in susceptible and resistant tomato (Lycopersicon esculentum Linn.) infected by the bacterial wilt pathogen (Pseudomonas solanacearum E. F. S.)

Date

3-1992

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Evelyn Mae T. Mendoza

Abstract

Biochemical changes in both resistant and susceptible tomato plants were analyzed in terms of changes proteins and changes in production of secondary Accession a 70, a resistant tomato plant, increased in total alkali-soluble proteins in its stalks by And its roots by 18.787. upon inoculation of the pathogen as shown by Lowry assay. Native gel electrophoresis showed three new band patterns which may be related to its mechanism of resistance against the bacterial wilt pathogen. accession # 508 exhibited an increase in total alkali soluble proteins only in the stalks by 24.24 % . For both accessions, there was a decrease in total alkali-soluble proteins in the leaves. Yellow Plum, a susceptible tomato plant, also Increased in total alkali-soluble proteins in the leaves by and in the stalks by 62.7% but decreased in the roots by 48.9% in response to pathogenic infection. Its electrophoregram showed the absence of some protein bands in the infected plants which is present in the control. Percent change in the degree of lignification was highest in the stalks of Accession 70 (32.24%) and in the leaves of Accession N 508 (53.73%). Delignification was observed in all plant parts of Yellow Plum. However, trypsin inhibitor and condensed tannins seemed not to be related in the resistance of tomato against bacterial wilt pathogen although these may be involved an resistance against other pathogens.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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