A comparative study on selected cultivated species of Philippine Medinilla using isozyme analysis

Date

4-2002

Degree

Bachelor of Science in Biology

Major Course

Major in Ecology Genetics

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Teresita L. Rosario

Abstract

Four cultivated species of Philippine Medinilla were evaluated in the study for isozyme polymorphism of three enzyme systems: acid phosphatase (ACPH), alkaline phosphatase (ALPH), and esterase (EST). Three replicates were used for Medinilla magnifica. Medinilla miniata and Medinilla venosa. Only one sample was utilized for Medinilla trianae. Results of electrophoresis showed six presumptive loci encoding the three enzymes. Two loci were observed from ACPH. ACPH-1 was observed to be controlled by two alleles, while ACPH-2 was controlled by three alleles. Five banding patterns were observed for this enzyme system. Two loci were also observed from ALPH. ALPH-1 was controlled by a single allele, while ALPH-2 was controlled by three alleles. Only three banding patterns were observed for this enzyme. Two loci were also found to encode EST. EST-1 was controlled by three alleles, while EST-2 was controlled by four alleles. EST-2 was observed to be dimeric. Six banding patterns were found in this enzyme. Out of the six loci encoding the three enzymes, only four were found to be polymorphic. Only the two ACPH and two EST loci were observed to be polymorphic. A 0.67-degree of polymorphism was arrived for the Medinilla species involved in the study. An average heterozygosity of 0.55 was also computed for the samples involved. High interspecific and intraspecific variation was also observed. Using the six-locus combination, all but two of the Medinilla samples were genotypically differentiated from each other.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

This document is currently not available here.

Share

COinS