Salt tolerance evaluation of thirty nine genotypes of rice (Oryza sativa L.) using morphological, pollen fertility, physiological and molecular characters during reproductive stage

Date

4-2007

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ivan Marcelo A. Duka

Co-adviser

Rakesh Kumar Singh

Abstract

Thirty nine genotypes of rice (Oryza sativa L.) were grown and evaluated for salinity tolerance during reproductive stage. The different genotypes were characterized morphologically to distinguish salt tolerant genotypes from salt sensitive ones based on pollen fertility, assess salt tolerance based on Na/K ratio in the reproductive parts of the rice plant (i.e. flag leaf, boot sheath, anther, and ovary) and group the selected genotypes based on molecular characters. Morphological characterization shows that TCA4 is the tallest variety (210 cm), and with the longest culm (166.167 cm). CN499 has the longest panicle (56.667 cm) and the most number of productive tillers (21). In the pollen fertility analysis, Kuatik Putik has the highest percent pollen fertility (83% and 85% for cut and intact treatments, respectively). In the Na/K analysis. ANOVA showed significant differences both for flag leaf and boot sheath among the different genotypes. But for the anther and ovary samples of the eleven genotypes. there are no significant differences among the genotypes or the treatments. Kala rata, CAC 615, Pokkali 15388, Bhura rata 4-10, CN 499 and IR 4630 are the most tolerant varieties with the flag leaf sample having the lowest Na/K ratio. SAL 411 was the most tolerant with boot sheath sample. For the ovary samples, all eleven genotypes fall under the susceptible range. Conversely, for anther samples SAL 503 is the most tolerant. Cluster analysis for morphological, pollen fertility and physiological characters of the thirty nine genotypes revealed six groups. Molecular characterization of the eleven genotypes using SSR markers revealed three clusters putting together IR64 and SAL 546 in one group; IR29, CSR 11 and 1R4630 in another, and MI-48. SAL 503. FL478, Chcriviruppu and IR28 in the third group.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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