Establishment of genetic markers for the segregation of Trichoderma reesei Rut C30 x Penicillium funiculosum Thom MG 171 fusants

Date

3-1991

Degree

Bachelor of Science in Biology

Major Course

Major in Ecology Genetics

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Saturnina C. Halos

Abstract

Fungicide, antibiotic and metal ion resistance were established as genetic markers for Trichoderna reesei Rut C30 and Penicilliun funiculosun Tho. KG 171. Segrega-tion patterns of resistance traits were determined from an analysis of their fusants and possible linkage relationships were suggested. Results indicate that P. funiculosuc Thoe KG 171 was resistant to 1 ppm mercury, 10 ppm arsenic and 50 ppm nystatin while growth of T. reesei Rut C30 was completely inhibited. On the other hand, T. reesei Rut C30 was re-sistant to benlate, cobalt, captan and vitigran blue at 1, 10, 25 and 1,500 ppm, respectively. There are inherent differences among the strains with respect to the number of colonies surviving even without the selective factors. There are significant differences among strains in all selective factors, but significant differences among selective factors and control are observed only in arsenic, nystatin, vitigran blue and benlate. Resistance to captain and vitigran blue as well as resistance to arsenic and nystatin tended to be inherited from the same parent suggesting a possible linkage between these traits.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 1991 B4 R43

Document Type

Thesis

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