"Electrophoretic investigation of total blood proteins of three species of fish collected from Naujan Lake, Oriental Mindoro : "ayungin", Therapon plumbeus (Kner), "dalag", Ophicephalus striatus (Bloch), and "hito", Clarias batrachus (Bloch)"

Date

3-1997

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ivan Marcelo A. Duka

Abstract

Blood protein banding patterns of three species of fish from Naujan Lake, “ayungin” (Therapon plumbeus [knet]), “dalag” (Ophicephalus striatus [Bloch]), and “hito” (Clarias battrachus [Bloch] were determined through Sodium-Dodecyl sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). This was done to investigate the number of blood protein bands of the three species, to describe in terms of relative mobilities and intensities the blood protein in each of these three species, and to compare blood protein banding patterns among these three species.

T. plumbeus had a total of 25 bands ranging from 10 to 20 bands for each sample. 0, striatus showed a total of 26 bands ranging from 14 to 23 bands for each sample. C. battachus had a total of 29 bands ranging from 10 to 25 bands for each sample. Due to the abundance of homoglabin in the blood, it was hypothesized that the thick and darkly stained bands are homoglabin polypeptide chains. These bands are band number 18, 14 and 21 in T. plumbeus 0. Striatus, and C. battachus, respectively. The rest of the bands which are also darkly stained could represent the other polypeptide subunits of other proteins present in the blood.

(Statistical analysis ) x2 Goodness-of-Fit Test) using total number of bands per sample as basis for comparison indicated that there are no significant differences between sample in each species and among the three species studied. This may indicate that the occurrence of heterogeneity is consistent within samples of one species and within different species exposed to a similar or one type of environment.

Low similarity index within population indicated that there exist biochemical variation at species level. Comparison between any combination of two of the three species showed a much lower similarity index. However, three were shown to be common to the three species. These hands are designated an bands A, B and C and corresponds to band numbers 6, 8, and 12 in T plumbeus band numbers 5, 8 and 11 in 0. Striatus; and band numbers 3, 5 and 10 in C. batrachus, respectively. Visualinspection of major bands revealed that they are almost homogenous which may be attributed to the absence of pollution in Naujan Lake.

The slight differences of banding patterns may be attributable to both environment and the inherent genotype differences of the samples studied.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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