Genetic diversity analysis of ivy ground, Coccinia grandis (L.) Voigt, using Random Amplified Polymorphic DNA (RAPD) markers

Date

10-2003

Degree

Bachelor of Science in Biology

Major Course

Major in Ecology Genetics

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ivan Marcelo A. Duka

Abstract

Random amplified polymorphic DNA (RAPD) markers were used to determine the degree of genetic diversity among the different accessions of a perennial dioecious and outcrossing species, Coccinia grandis (1..) Voigt, collected from the different provinces of Thailand and kept at the Asian Vegetable Research and Development Center (AVRDC). Sixty-two samples consisting of 35 female and 27 male samples were analyzed using five random primers. A total of 85 RAPD bands were scored for all accessions. No sex-specific markers were noted but accession specific markers were observed in male samples of accessions CGr038 and COr043 and in female samples of accessions CGr001, CGr010. CGrOI 1, CGr012, CGr016, CGr024, CGr025, CGr033 and CGr038. Calculation of genetic diversity using Shannon information index revealed that 89% of variation occurs within male and female groups while only 11% of genetic diversity occurs between male and female groups. Jaccard's similarity values ranged from 0.196 to 0.920 for all samples indicating a wide range of genetic diversity. The UPGMA-based dendrograms for both male and female samples exhibit short internode branching indicating instability of clusters. No confidence limits 95% or higher was observed among the two majority-rule consensus trees formed by bootstrap analysis. Only a few accessions grouped according to geographic origin while some accessions were found to be more similar to accessions coming from different geographic origins. The low similarity values, instability of the internode branches of the UPGMA-based dendrograms, low bootstrap confidence limits and high measure of diversity using Shannon's information index within male and female groups suggest a high level of genetic diversity within the C grandis germplasm collection. It is therefore advisable that more primers be used to confirm the results and to provide more markers for more information of similarity measures between accessions.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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