Karyotype analysis of in vitro cultured human leukocytes treated with crude leaf extract of chichirica (Catharanthus roseus L.)

Date

3-1998

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Abstract

Photomicrographs of cells from slides of human leukocytes cultured in vitro treated with varying concentrations of the crude leaf extract of catheranthus roseus L. were obtained karyograms of each of the cells were made. The relative length and the slope of the average relative lengths of each chromosomes were obtained. The data was also subjected to analysis of variance. Results showed that chromosomes 1, 2, 4, 6, 7, 8, 9 and 15 have negative slopes, all others with positive slopes. A negative slope indicates a decrease in the relative length of the chromosome. There were also significant differences among the treatment groups for chromosomes 2, 3, 6, 14, 16, and 21. The results indicate that Catheranthus roseus L. leaf extract has components that can cause chromosome condensation and decondensation.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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