Protein polymorphism in domesticated populations of the stingless bee Trigona biroi Friese from Laguna, Cavite, and Quezon

Date

4-2007

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Dennis B. Bela-Ong

Abstract

lntraspecific variation in Trigona biroi F. was assessed based on two hemolymph proteins, namely: transferrin (TRF) and globulin (GLO) and three enzyme systems, namely: acid phosphatase (ACPH), esterase (EST), and malic enzyme (ME) using native polyacrylamide gel electrophoresis. Hemolymph protein banding patterns showed a total of five presumptive loci while isozyme banding patterns revealed a total of six presumptive loci. One TRF and four GLO loci were all monomorphic with TRF being a dimeric protein. ACTPH and EST had several loci: two isoloci for ACPH and three for EST. Only one locus was detected in ME. The degree of genetic variability in the three populations of T. biroi was determined by calculating the proportion of polymorphism (P), average number of alleles per locus (A), average heterozygosity (H). genetic identity (I), genotypic similarity (S), and genetic distance (D). Low genetic variability was observed in the three populations as shown by the low P values (40%-45.45%) and low H values (17.77%-25.85%). Based on all loci. T. biroi from Laguna and Cavite had approximately one allele per locus while approximately two alleles per locus in T hiroi from Quezon. I and S were highest in Laguna and Cavite (1=0.983, S=0.945) followed by Laguna and Quezon (1=0.911, S=0.899). and Cavite and Quezon (1=0.905, S=0.874). On the other hand, genetic distance was highest in Cavite and Quezon (D=0.100) followed by Laguna and Quezon (D=0.093), and Laguna and Cavite (D=0.018). A dendrogram derived from gene frequencies indicated that populations from Laguna and Cavite were 100% similar to each other whereas Quezon populations were only 79% similar to Laguna and Cavite populations.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

This document is currently not available here.

Share

COinS