Protein polymorphism among populations of beef cattle (Bos indicus) from Pangasinan, Tarlac, Romblon and Siquijor, Philippines

Date

4-2004

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Rita P. Laude

Abstract

Isozyme variation in beef cattle (Box indicus) from Pangasinan, Tarlac. Romblon. and Siquijor was investigated. A total of 103 plasma samples was analyzed for blood proteins namely, albumin (Alb) and transferrin (Tel), and isozymes such as esterase (Est), acid phosphatase (Acph), and malate dehydrogenase (MDII) using starch gel electrophoresis. Polymorphism in all the nine presumptive loci except for Alb, Est, Acph,. and MDH2 was noted. In all four locations considered, albumin showed only one banding pattern corresponding to homozygous genotype AIb AA, which is assumed to be controlled by a single gene designated as AIb A. Five genotypes of transferrin, Trf AA, Trf CC. Trf AB, Trf AC, and Trf BC were identified with Trf AC as the predominant genotype. Transferrin genotypes were controlled by Trf A, Trf B, and Trf C genes. Esterase showed three loci: Est,. Este which both gave three kinds of genotypes (AA, AB, and BB). and Est, which exhibited only one genotype (Est, AA). Est, locus was controlled by Est, A and Est, B genes. Similarly, Este locus was coded by Este A and Est2B genes. Est,, on the other hand, was controlled by Est, A. Two loci were observed in acid phosphatase: Acph,, which gave only one genotype (Acph, AA), and Acph2, which exhibited three genotypes (Acph2 AA, Acph2 AB, and Acph2 BB). Acph, A, Acph2 A. and Acph2 B genes were assumed to control acid phosphatase genotypes. Two loci were observed in malate dehydrogenase: MDI I, and MDH2. MD11, had three genotypes (MD1-1,. AA. MD11, BB, and MDI-1, AB), while MDH2 locus had MDH2 AA genotype only. MDI-I, genotypes were controlled by genes MD11, A and MDI-1, B, while MDH2 was coded by gene MDH2 A. Intrapopulational studies revealed highest heterozygosity (H) value of 0.324 in the cattle population from Tarlac followed by Siquijor, Romblon, and Pangasinan with 0.223, 0.196. and 0.164. respectively. The proportion of polymorphic loci (P) was found to be the same for the three locations studied (Tarlac. Romblon, and Siquijor) having a value of 0.556, while Pangasinan had a different value of 0.444. The average number of alleles per locus (A) ranged from 1.667-1.778. Analyses on interpopulation variations showed low genetic identity (0.7701) and high genetic distance (0.2612) between Siquijor and Romblon cattle. This indicated low degree of relatedness between these two populations. On the other hand, beef cattle from Tarlac and Siquijor had the highest genetic identity (0.9161) and lowest genetic distance (0.0876) showing highest degree of relatedness, hence shared many genes in common.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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