Comparative electrophoretic analysis of amylases from hemolymph of golden snails (Pomaceae sp.) and native kuhol (Pila luzonica Reeve)

Date

3-1989

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Desiree M. Hautea

Abstract

Isozyme banding patterns of amylase from Pomaceae sp. and Pila luzonica Reeve hemolymphs were determined using starch-acrylamide gel electrophoresis. Results showed three amylase bands (AMY 1, IY 2, AMY 3) characterized by three migration zones in the gel and ore band patterns (I, II, III). AMY 1, was the slowest migrating cd (rf value = 0.0495). AMY 2 hand was a fast migrating band with , Rt = ti.0770. AMY 3 was the fastest migrating band with an Rf =0.0995. The three banding patterns, observed consisted of the . +Mowing wands: pattern I has AMY 1; pattern II has both AMY 1 and .0!Y 2 and pattern III has AMY 1 and AMY 3. Both snail species exhibited two amylase bands and two banding patterns. AMY 1 band was present in both Pomaceae sp. and P. a7cnica with a frequency of 100% for all the individual samples he two ,peices differed in the second band wherein Pomaceae sp. inhibited AMY 3 whereas P. luzonica showed AMY 2. In terms of band pattern observed, both snails exhibited pattern 1, however, their freqencies oi occurrence differed. The second band pattern also differed in the two snails. pomaceae sp. exhibited pattern III while Pila. luzonica exhibited pattern II.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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