Response of Dendrobium hybrids to different concentrations of colchicine applied at different developmental stages of growth

Date

4-1994

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Teresita L. Rosario

Abstract

The response of Dendrobium hybrid seedlings to difference concentrations of cochicine (0.0%, 0.01%, 0.05%, 0.1 and 0.2%) applied at different developmental stages of was investigated. Cochicine was applied by drop me, h,0 to newly single-potted seedlings of Dendrobium Thomas Pink' X Dendrobium 'Sonia' in Experiment I, was Incorporated in the artificial media in Experiment whet, ,-.even-month old Dendrobium 'Jacquelyn Thomas Pink seedlings, were reflasked. in general, treated seedlings have shorter, greener .end as the concentration of colchicine is increased decrease in needling height, leaf number and shoot number observed. Statistical analysis showed that only seed treated with 0.01% have significantly thicker leaves. Inure were also significant differences in stomatal density and pore length among the treatments. On the other hand, no abnormalities in and mitosis of the treated and untreated 1.-int, were observed. In Experiment 2, limited observations were made due to the earl, browning of the seedlings which eventually led to their death. The treated plants were shorter because of the threatening of their leaf base. Tumor-like bodies were also observed. The above results showed that colchicine affects several seedling characteristics. Further studies should be made when the plants have matured and produce flowers to determine the true effect of colchicine.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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