Detection of aucubin in the ocal Plantago major L. (Plantaginaceae) and induction of multiple shooting using Thidiazuron

Date

4-2008

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Lourdes B. Cardenas

Abstract

Aucubin, the key biologically active compound in this species, was detected by thin - layer chromatography (TLC) in the leaf extracts of both wild and in vitro cultures of local Plantago major. TLC bands specific for iridoid glycosides like aucubin were detected at Rf 0.45 - 0.47 (Wagner and Bladt, 1996). Morphological observations of both wild and in vitro P. major plants did not show variations except for a more robust growth and pronounced green color of the wild plant. Duc to the limited source of this plant material, P. major seeds were aseptically germinated and multiple shooting was induced using Thidiazuron (TDZ). Of the four TDZ treatments used in the range of 0 - 2 ppm, 1.3 ppm TDZ at 3 'A months exposure proved optimum for multiple shooting.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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