A comparative study on the protein profiles of the eye and muscle tissues in three varieties of angelfish Pterophyllum scalare heckel using Sodium Dodecyl Sulphate- PolyAcrylamide Gel Electrophoresis (SDS-Page)

Date

3-2001

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ivan-Marcelo A. Duka

Abstract

The variation of the protein profiles in the eye and muscle tissues of three varieties of angelfish namely marble, plain and wild type were analyzed and compared using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Results show a total of 20 bands resolved in the eye samples. The plain angelfish had the most number of band patterns (20). Each sample of marble angelfish exhibited its own band pattern. Band numbers 1, 2, 5, 8, 9, 10, II, 14, 15, and 18 are the common bands observed in the eye samples of the three varieties of angelfish studied. For the muscle tissues. 23 bands were resolved. The marble variety had the most variable band pattern (20). The wild type variety showed only two hand patterns. We can therefore say that the samples of wild type angelfish were almost uniform in terms of their protein bands. The common bands observed in the muscle samples of three varieties of angelfish are band numbers 1. 2. 3, 4, 6, 7, 8, 12, 13, and 14. Based on the index of similarity computed, there is a considerable amount of similarity in the protein bands of the eye tissue between the plain and wild type varieties. On the other hand, no significant difference in the protein bands of the muscle tissues exists among the three varieties of angelfish. The variation observed in the protein profiles of the eye and muscle tissues of the three varieties of angelfish may be due to the absence and presence of proteins. mutation and variable developmental stages of the samples.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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