Genetic variation in populations of European honeybee, Apis mellifera carnica P. from selected apiaries using Isozyme analysis

Date

4-2007

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Hazel L. Gaza

Abstract

Eight presumptive loci were detected in three populations of Apis melliftra (carnica P. from Los Banos, Bicutan and Cavite apiaries using starch gel electrophoresis. The isoloci detected are acid phosphatase (ACPH-I and ACPH-2), alkaline phosphatase (ALPH-1 and ALPH-2) and esterase (EST-I, EST-2, EST-3 and EST-4). Three bands in polymorphic ACPH-I locus was observed namely S (0.057-0.094), M (0.113-0.189). and F (0.189-0.245). Meanwhile. ACPH-2 was controlled by only two alleles. S (0.245-0.302) and M (0.321-0.377). Bicutan and Cavite are both monomorphic, while Los Banos was polymorphic. ALPH-I. possesses three alleles with the following values: 0.057-0.151 for S. 0.189-0.245 for M, and 0.245-0.302 for F. Bicutan and Los Banos populations were polymorphic while Cavite was monomorphic. Monomorphic ALPII-2 was only present in Cavite with a single allele S (Rf = 0.302-0.359). Four monomorphic esterase loci were discovered all controlled by a single allele F with the following Rf values: 0.076-0.132. 0.170-0.208, 0.245-0.302, and 0.377-0.434. EST/ in Bicutan and Cavite. EST-2 in Cavite only, EST-3 in all populations and EST-4 in Bicutan only. Gcnctic variation within population is low. This is evident in the low proportion of polymorphic loci ranging from 0.125-0.375. low heterozygosity values ranging from 0.082-0.234 and average number of alleles per locus values approaching 1.000. The results observed could be due to non-random mating. Genetic variation between Cavite and Bicutan is low. This is seen in the low genetic distance of 0.40 and high genotypic similarity value of 0.673. This is in contrast to Los Banos and Cavite populations with genotypic similarity of 0.385 and genetic distance of (0.71). The high genetic variation detected between these two populations was due to different method of rearing queens for establishment of new colonies.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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