Detection of 1- AminoCyclopropane-1- Carboxylate (ACC) synthase mRNA in coconut endosperm (Cocos nucifera)

Date

3-1999

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Evelyn Mae T. Mendoza

Abstract

Despite the abundance of studies conducted on ethylene and the enzyme, which is most essential to its biosynthesis. l-aminocyclopropane-l-carboxylate (ACC) synthase, no studies have ever been conducted using coconut endosperm as a system Furthermore, of the mutant phenotype, investigations at the molecular level of the mutant phenotype of coconut still need to be conducted to gain a lull understanding of the phenomenon In line with this, detection of the ACC synthase transcripts in 6 month normal and mutant coconut endosperm was conducted using Reverse Transcription-Polymerase Chain Reaction ACC synthase gene was expressed in the endosperm of the 6 mo normal nut but not at older stages such as 8 and 9 mo Polyamines accumulate in normal endosperm during maturation, which inhibits ACC synthase transcription The ACC synthase gene was also not expressed in the mutant endosperm, since cytokinins accumulate in this tissue, whose presence also inversely inhibits ethylene production

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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