Protoplast isolation from the spindle tissue of sugarcane, Saccharum officinarum L.

Date

6-1981

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Liwayway M. Engle

Abstract

Protoplasts were isolated from the spindle tissue of Saccharum officinarum using an enzyme mixture of cellulase, sorbitol and magnesium chloride. The effects of cellulase concentration, pH and sorbitol concentration were investigated. Cells at different stapes of cell wall degradeticn were observed. Protoplasts isolated were irregularly shaped due to the high sugar concentration used as storing medium. Optimum results were obtained at 4% cellulase, pH 5.0 and 0.5 M sorbitol.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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