Partial molecular characterization of Free Nucleic Acids (FNA) from the water of aromatic dwarf variety of coconut(Cocos nucifera L.)

Date

4-2006

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ivan Marcelo A. Duka

Abstract

This study primarily aims to isolate and analyze Free Nucleic Acids (FNA) from the water of aromatic dwarf variety of coconut (Cocos nucifera L.). Degradation treatments using DNase I and RNase A, followed by a colorimetric test confirmed that FNA in coconut water (CW) contains both RNA and DNA. Further characterization was done using Anion Exchange Purification Techniques with the use of an Oligo (dT) cellulose powder and a silica gel matrix. After a colorimetric test, it was found out that the FNA in CW is mainly dominated by single-stranded mRNA species and traces of double-stranded FNAs. Since FNA might be involved in endosperm development, the changes in concentration of FNA in coconut fruit development were distinguished. The concentration of FNA in the developing fruit showed an increasing trend which may indicate a possible correlation with endosperm development. The availability of mRNA in CW gives rise to the possibility of cDNA synthesis to further confirm and verify the role of mRNA in gene expression during endosperm development.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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