Assessment of genetic diversity of beef cattle (Bos indicus Linn.) in different small holder production systems in Ilocos Norte and Isabela using protein/isozyme analysis

Date

4-2004

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Rita P. Laude

Abstract

The genetic diversity of cattle (Bos indicus Linn.) from !locos None and Isabela was determined using protein/isozyme analysis. A total of 59 blood samples were collected and analyzed. Six presumptive loci were detected which includes acid phosphatase (Acph), albumin (Alb), esterase 1 (Est,), esterase 2 (Est,), malic dehydrogenase (Mdh), and trasferrin (Trf). Four of these six presumptive loci were polymorphic which includes; (I) Acph, Esti, Mdh. and Trf. The remaining two which were classified as monomorphic were Est, and Alb. Three genotypes were exhibited by Acph (AA. AB, BB), Est, (AC, CC, BC). and Mdh (AA, AB. BB) . Trf exhibited the most numerous genotypes (AB, AC, BB, BC, and CC). Intrapopulational variability indices revealed that !locos None cattle populations were slightly higher in heterozygosity (H) (38%) than the Isabela populations (24%). The number of polymorphic loci (P) was the same in both populations (0.66). The average number of alleles per locus (A) in 'locos None cattles was higher (2.0) than the Isabela cattles (1.67). A high value of genetic identity (0.93) occurred between cattle populations of (locos None and Isabela. A high degree of genetic relatedness and genetic closeness between the two populations was indicated by the low genetic distance (D) of 0. 073. Thus, the two cattle populations studied were considered genetically related.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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